The more the merrier: how homo-oligomerization alters the interactome and function of ribonucleotide reductase
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Combinatorial mutagenesis was used to investigate the role of three key residues in cytochrome c peroxidase (CCP) from Saccharomyces cerevisiae, Arg48, Trp51, and Trp191, in control of the reactivity and selectivity of the heme-contg. enzyme. Libraries wer ...
Genes integrated near the telomeres of budding yeast have a variegated pattern of gene repression that is mediated by the silent information regulatory proteins Sir2p, Sir3p, and Sir4p. Immunolocalization and fluorescence in situ hybridization (FISH) revea ...
We describe two general methodologies, based on filter-sandwich assays, for isolating enzymic activities from a large repertoire of protein variants expressed in the cytoplasm of E. coli cells. The enzymes are released by the freezing and thawing of bacter ...
Oxygen is a key substrate in animal cell metab. and its consumption is thus a parameter of great interest for bioprocess monitoring and control. A system for measuring it based on an oxygen balance on the liq. phase was developed. The use of a gas-permeabl ...
In budding yeast, a switch between the mutually exclusive pathways of cell cycle progression and conjugation is controlled at Start in late G1 phase. Mating pheromones promote conjugation by arresting cells in G1 phase before Start. Pheromone-induced cell ...
The aim of the invention is to improve existing amperometric sensors used to measure the quantity of a component present in a solution. This aim is reached by using a sensor provided with a measuring electrode (20) having at least one current collector (37 ...
A method and app. for enzymic hydrolysis of proteins comprises a 1st vessel in which the substrate and enzyme is mixed and the substrate undergoes a 1st hydrolysis. This hydrolyzate is subjected to a 2nd hydrolysis step in a tube or tubes contg. stationary ...
Enzymic hydrolyses of naturally occurring protein sources represent complex reactions at a molecular level because of the specificity pattern of proteases and the composition of the substrates involved. In addition, rather global criteria are in practical ...
Poly(A) polymerase was purified 22,000-fold to homogeneity from a whole cell extract of Saccharomyces cerevisiae with a yield of 22%. The enzyme is a monomeric polypeptide with a denatured molecular weight of 63,000. Incorporation of labeled ATP into acid- ...
Vibrio and Aeromonas spp. secrete an unusual 35-kDa lipase that shares several properties with mammalian lecithin-cholesterol acyltransferase. The Aeromonas hydrophila lipase contains two cysteine residues that form an intramolecular disulfide bridge. Here ...
