Publication

Label-free, scalable and point-of-care imaging platform for rapid analysis of biomarker

Résumé

We propose a disruptive point-of-care (PoC) imaging platform based on lens-free interference phase-contrast imaging for rapid detection of biomarker such as for sepsis and potentially other diseases (e.g. cancer). It enables simultaneous analysis of potentially up to 10,000 functionalized microarray spots with different biomarkers with fast time-to-results (few minutes) and by consuming a small sample volume (similar to 10 mu L). The high sensitivity allows direct measurements of the biomarker binding without the use of fluorescent labels (e.g. ELISA) or microbial culture methods. In addition, ad-hoc plasmonic nano-structuring is utilized to significantly improve the sensitivity for biomarker detection (optical path difference similar to A) to concentration levels relevant for disease diagnosis. The proposed technology incorporates a portable and low-cost lens-free imaging reader made of consumer electronic components, plasmonic microarrays with distinct functionalization, and user-friendly software based on a novel phase-shifting interferometry method for topography and refractive index analysis. Due to its compactness and cost-efficiency, we foresee a great potential for PoC applications, especially for the rapid detection of infectious diseases or life-threatening conditions, e.g. sepsis, but also for clinical trials of drugs and food control.

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Concepts associés (34)
Phase-contrast imaging
Phase-contrast imaging is a method of that has a range of different applications. It measures differences in the refractive index of different materials to differentiate between structures under analysis. In conventional light microscopy, phase contrast can be employed to distinguish between structures of similar transparency, and to examine crystals on the basis of their double refraction. This has uses in biological, medical and geological science.
Phase-contrast X-ray imaging
Phase-contrast X-ray imaging or phase-sensitive X-ray imaging is a general term for different technical methods that use information concerning changes in the phase of an X-ray beam that passes through an object in order to create its images. Standard X-ray imaging techniques like radiography or computed tomography (CT) rely on a decrease of the X-ray beam's intensity (attenuation) when traversing the sample, which can be measured directly with the assistance of an X-ray detector.
Interférométrie
vignette|Le trajet de la lumière à travers un interféromètre de Michelson. Les deux rayons lumineux avec une source commune se combinent au miroir semi-argenté pour atteindre le détecteur. Ils peuvent interférer de manière constructive (renforcement de l'intensité) si leurs ondes lumineuses arrivent en phase, ou interférer de manière destructive (affaiblissement de l'intensité) s'ils arrivent en déphasage, en fonction des distances exactes entre les trois miroirs.
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Publications associées (36)

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Microscopic visualisation of optically transparent samples has been a topic of interest for several decades. Features such as density or chemical composition can influence the optical phase of transmitted light, and phase contrast can reveal these structur ...
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Direct visualization is often sought to elucidate flow patterns and validate models to predict the filling kinetics during processes whereby a liquid resin infiltrates a textile porous preform. Here, X-ray phase contrast interferometry is evaluated to imag ...
2021

Multi-spectral image priniting method

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The present disclosure concerns a multi-spectral image printing method. The method includes the steps of: - providing a multi-spectral image; - providing a material; - determining changes in a refractive index value of the material permitting to reproduce ...
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