High-throughput and adaptive super-resolution fluorescence microscopy of organelles
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Fluorophores that are fixed during image acquisition produce a diffraction pattern that is characteristic of the orientation of the fluorophore’s underlying dipole. Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolu ...
Infrared scanning near-field optical microscopy (IR-SNOM) is an extremely powerful analytical instrument since it combines IR spectroscopy's high chemical specificity with SNOM's high spatial resolution. In order to do this in the infrared, specialty chalc ...
The present thesis develops some specific aspects of digital holographic microscopy (DHM), namely the effect of shot noise on the phase image accuracy, the use of DHM in micro-tomography and in aberrations evaluation of a microscope objective (MO). DHM is ...
We report on polarization sensitive imaging of two-dimensional photonic crystal microcavity modes. By using a near-field scanning optical microscope with a polarization sensitive setup, it is possible to selectively map, with a resolution beyond the diffra ...
Ptychography is a coherent diffractive imaging method that uses multiple diffraction patterns obtained through the scan of a localized illumination on the specimen. Until recently, reconstruction algorithms for ptychographic datasets needed the a priori kn ...
We present a systematic numerical investigation of conical metal tips which are commonly used in tip-enhanced Raman spectroscopy (TERS). Different from previous studies, we focus on how the tip length and the illumination condition influence the local fiel ...
We report fluorescence correlation spectroscopy (FCS) measurements using near-field scanning optical microscopy (NSOM) probes to produce a sub-diffraction-limited observation area. An order of magnitude reduction in the area compared to confocal FCS has be ...
Due to the limited depth of field of brightfield microscopes, it is usually impossible to image thick specimens entirely in focus. By optically sectioning the specimen, the in-focus information at the specimen's surface can be acquired over a range of imag ...
We introduce a novel microscopy method of probing the spatial frequencies of a two dimensional sample with a single point detector allowing for sub- micron resolution with low NA lenses as well as for lateral phase resolution in the nanometer range. We syn ...
We introduce an efficient, image formation model-based algorithm that extends super-resolution fluorescence localization to include orientation estimation, and report experimental accuracies of 5 nanometers for position estimation and 2 degrees for dipole ...