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Influence of Detergent and Lipid Composition on Reconstituted Membrane Proteins for Structural Studies

Publications associées (43)

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Molecular-level characterization of curved water interfaces with sum frequency and second harmonic scattering

Nikolay Smolentsev

Aqueous interfaces are omnipresent in nature. Some of them are visible: droplets in clouds, aerosols, and surfaces of oceans, lakes, and rivers. Many more surfaces are hidden: water in contact with cell membranes, proteins, and the surface of droplets in e ...

EPFL2017

The Structure of the Mouse Serotonin 5-HT3 Receptor in Lipid Vesicles

Horst Vogel, Henning Paul-Julius Stahlberg, Cédric Deluz, Luigino Grasso, Ghérici Hassaïne

The function of membrane proteins is best understood if their structure in the lipid membrane is known. Here, we determined the structure of the mouse serotonin 5-HT3 receptor inserted in lipid bilayers to a resolution of 12 Å without stabilizing antibodie ...

Elsevier2016

Here we present a planar patch clamp chip based on biomimetic cell membrane fusion. This architecture uses nanometer length-scale surface patterning to replicate the structure and function of membrane proteins, creating a gigaohm seal between the cell and ...

Nature Publishing Group2016

The human voltage dependent anion channel 1 (VDAC) is a 32 kDa β-barrel integral membrane protein that controls the transport of ions across the outer mitochondrial membrane. Despite the determination of VDAC solution and diffraction structures, a structur ...

2015

3D reconstruction of two-dimensional crystals

Henning Paul-Julius Stahlberg, Andreas Engel

Electron crystallography of two-dimensional (2D) crystals determines the structure of membrane proteins in the lipid bilayer by imaging with cryo-electron microscopy and image processing. Membrane proteins can be packed in regular 2D arrays by their recons ...

Elsevier BV2015

Perturbations of the Straight Transmembrane alpha-Helical Structure of the Amyloid Precursor Protein Affect Its Processing by gamma-Secretase

Matteo Dal Peraro, Patrick Fraering, Mitko Dimitrov, Thomas Lemmin

Background: Amyloid- neurotoxicity depends on the specificity of the proteolytic cleavage of the amyloid precursor protein (APP) transmembrane domain. Results: The APP transmembrane -helix is straight in a biological membrane bilayer. Conclusion: The flexi ...

American Society for Biochemistry and Molecular Biology2014

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P4-ATPases (flippases) translocate specific phospholipids such as phosphatidylserine from the exoplasmic leaflet of the cell membrane to the cytosolic leaflet, upholding an essential membrane asymmetry. The mechanism of flipping this giant substrate has re ...

Natl Acad Sciences2014

Refining Mitochondria Segmentation in Electron Microscopy Imagery with Active Surfaces

Pascal Fua, Anne Alison Jorstad

We present an active surface-based method for refining the boundary surfaces of mitochondria segmentation data. We exploit the fact that mitochondria have thick dark membranes, so referencing the image data at the inner membrane can help drive a more accur ...

Springer2014

Over the past years, molecular modeling and simulation techniques have had a major impact on experimental life sciences. They are capable of providing accurate insight into microscopic mechanisms, which are usually difficult to investigate experimentally. ...

EPFL2013

This article discusses future perspectives of solution NMR spectroscopy to study structures and functions of integral membrane proteins at atomic resolution, based on a review of recent progress in this area. Several selected examples of structure determin ...

Taylor & Francis2013