Publication
In vitro endoderm emergence and self-organisation in the absence of extraembryonic tissues and embryonic architecture
Publications associées (157)
Accessible and highly observable gastrointestinal organoid model systems for studying host-pathogen interactions
Traditional cell cultures have long been fundamental to biological research, offering an alternative to animal models burdened by ethical constraints and procedural intricacies, often lacking relevance to human physiology and disease. Moreover, their inabi ...
EPFL2024Programming of Multicellular Patterning with Mechano-Chemically Microstructured Cell Niches
Multicellular patterning of stem-cell-derived tissue models is commonly achieved via self-organizing activities triggered by exogenous morphogenetic stimuli. However, such tissue models are prone to stochastic behavior, limiting the reproducibility of cell ...
WILEY2023Neuronal migration prevents spatial competition in retinal morphogenesis
The concomitant occurrence of tissue growth and organization is a hallmark of organismal development(1-3). This often means that proliferating and differentiating cells are found at the same time in a continuously changing tissue environment. How cells ada ...
NATURE PORTFOLIO2023Multimodal characterization of murine gastruloid development
Matthias Lütolf, Alexandre Gauthier Aurèle Mayran, Stefano Davide Vianello, Raphaël Ortiz
Gastruloids are 3D structures generated from pluripotent stem cells recapitulating fundamental principles of embryonic pattern formation. Using single-cell genomic analysis, we provide a resource mapping cell states and types during gastruloid development ...
CELL PRESS2023How are cells instructed to form somite boundaries by the zebrafish segmentation clock?
In vertebrate embryos, the elongating body axis is patterned via the sequential and rhyth-mic production of segments from a posterior unsegmented tissue called the presomitic mesoderm (PSM). This process is controlled by a population of cellular oscillator ...
EPFL2023The nuclear-to-cytoplasmic ratio drives cellularization in the close animal relative Sphaeroforma arctica
Omaya Pierre Dudin, Marine Olivetta
The ratio of nuclear content to cytoplasmic volume (N/C ratio) is a key regulator driving the maternal-to -zy-gotic transition in most animal embryos. Altering this ratio often impacts zygotic genome activation and de-regulates the timing and outcome of em ...
CELL PRESS2023Differential impact of TGF beta/SMAD signaling activity elicited by Activin A and Nodal on endoderm differentiation of epiblast stem cells
Allocation of cells to an endodermal fate in the gastrulating embryo is driven by Nodal signaling and consequent activation of TGF beta pathway. In vitro methodologies striving to recapitulate the process of endoderm differentiation, however, use TGF beta ...
WILEY2022An automated do-it-yourself system for dynamic stem cell and organoid culture in standard multi-well plates
Matthias Lütolf, Sebastian Maerkl, Hao-An Hsiung, Zoe Newell Swank, Stefano Davide Vianello, Julia Tischler
We present a low-cost, do-it-yourself system for complexmammalian cell culture under dynamically changing medium formulations by integrating conventional multi-well tissue culture plates with simple microfluidic control and system automation. We demonstrat ...
2022Deconstructing body axis morphogenesis in zebrafish embryos using robot-assisted tissue micromanipulation
Andrew Charles Oates, Mahmut Selman Sakar, Ece Özelçi, Erik Mailand, Matthias Rüegg
Classic microsurgical techniques, such as those used in the early 1900s by Mangold and Spemann, have been instrumental in advancing our understanding of embryonic development. However, these techniques are highly specialized, leading to issues of inter-ope ...
2022Organoids as tools for fundamental discovery and translation-A Keystone Symposia report
Complex three-dimensional in vitro organ-like models, or organoids, offer a unique biological tool with distinct advantages over two-dimensional cell culture systems, which can be too simplistic, and animal models, which can be too complex and may fail to ...
WILEY2022