Publication
Identification of Isomeric Biomolecules by Infrared Spectroscopy of Solvent-Tagged Ions
Publications associées (45)
Ion Spectroscopy Reveals Structural Difference for Proteins Microhydrated by Retention and Condensation of Water
Protein ubiquitin in its +7 charge state microhydrated by 5 and 10 water molecules has been interrogated in the gas phase by cold ion UV/IR spectroscopy. The complexes were formed either by condensing water onto the unfolded bare proteins in a temperature- ...
Washington2024Bridging Native and Intrinsic Structures of Microhydrated Biomolecules by Cold Ion Spectroscopy
Solving native structures of such large molecules, like biomolecules, is often challenging, particularly due to the potentially infinite number of non-covalent interactions with water. In this thesis, we report the use of cold ion gas-phase action spectros ...
EPFL2023Advancing Glycan Analysis: Cryogenic Ion Spectroscopy for Database-Driven Identification
Glycans play a pivotal role in both physiological processes; however, the field of glycobiology remains relatively understudied within biochemistry. The intricate complexity of glycans, coupled with their abundance of isomeric forms, presents formidable ch ...
EPFL2023Standard-Free Quantification of Dicarboxylic Acids: Case Studies with Salt-Rich Effluents and Serum
Christian Ludwig, Daniil Salionov, Sasa Bjelic
The present study evaluates the ionization efficiency (IE) of linear and branched C2–C14 dicarboxylic acids (DCAs) by electrospray ionization (ESI) under different conditions. The influence of the concentration of organic modifier (MeOH); mobile phase addi ...
2022High-frequency gaseous and particulate chemical characterization using extractive electrospray ionization mass spectrometry (Dual-Phase-EESI-TOF)
Andrea Baccarini, Imad El Haddad, Mihnea Surdu, Houssni Lamkaddam
To elucidate the sources and chemical reaction pathways of organic vapors and particulate matter in the ambient atmosphere, real-time detection of both the gas and particle phase is needed. State-of-the-art techniques often suffer from thermal decompositio ...
COPERNICUS GESELLSCHAFT MBH2022Fragment ion-functional group relationships in organic aerosols using aerosol mass spectrometry and mid-infrared spectroscopy
Satoshi Takahama, Imad El Haddad, Nikunj Dudani, Amir Yazdani
Aerosol mass spectrometry (AMS) and mid-infrared spectroscopy (MIR) are two analytical methods for characterizing the chemical composition of OM. While AMS provides high-temporal-resolution bulk measurements, the extensive fragmentation during the electron ...
European Geosciences Union2022A gas phase approach for glycan analysis: Combining ultrahigh-resolution ion mobility spectrometry with cryogenic vibrational spectroscopy
The last decades have been punctuated by the development of revolutionary analytical technologies that have enabled the analysis of biomolecular structures and unraveled the roles they play in biological systems. However, certain classes of biomolecules re ...
EPFL2021Photoisomerization of Linear and Stacked Isomers of a Charged Styryl Dye: A Tandem Ion Mobility Study
The photoisomerization behavior of styryl 9M, a common dye used in material sciences, is investigated using tandem ion mobility spectrometry (IMS) coupled with laser spectroscopy. Styryl 9M has two alkene linkages, potentially allowing for four geometric i ...
AMER CHEMICAL SOC2021LC-APCI(-)-MS Determination of 1-Chloro-2,4-dinitrobenzene, a Model Substrate for Glutathione S-Transferases
Kristin Schirmer, Alena Tierbach
1-Chloro-2,4-dinitrobenzene (CDNB) is widely used as a model substrate for measuring the enzyme activity of glutathione S-transferases in toxicity studies and in studies focusing on the metabolic capacity of different test systems. To allow the quantificat ...
AMER CHEMICAL SOC2020Combining Ion Mobility and Cryogenic Spectroscopy for Structural and Analytical Studies of Biomolecular Ions
Thomas Rizzo, Michael Zachary Kamrath
Ion mobility spectrometry (IMS) has become a valuable tool in biophysical and bioanalytical chemistry because of its ability to separate and characterize the structure of gas-phase biomolecular ions on the basis of their collisional cross section (CCS). It ...
2018