Development of an Automated Total Nitrosamines (TONO) Analyzer

Formation of N-Nitrosamines in water and wastewater treatment is of concern due to their high carcinogenic potency, e.g., N-nitrosodimethylamine (NDMA) is estimated to be several hundred times more potent than the regulated trihalomethanes in drinking water. Rather than a direct release from specific sources, N-nitrosamines are known to be mainly formed in situ during disinfection processes such as chlorination, ozonation, etc. They are categorized as disinfection byproducts (DBPs). In principle, nitrosation can take place with any nitrogen-containing organic precursor present in water with a potential formation of a variety of N-nitrosamines. However, robust analytical tools available to date are somewhat limited to the commonly known individual N-nitrosamines, e.g., NDMA. In 2017, Breider and von Gunten developed an analytical platform which, based on UV photolysis and chemiluminescence, determines the total content of N-nitrosamines in water samples.1 This system had many shortcomings and therefore, the TONO analyzer is currently upgraded, aiming for a complete automation of the analytical procedure from sampling to detection including data analysis without human intervention. All the hardware modules such as autosampling platform, syringe pumps, valves, photoreactor, etc. were 3D-designed, 3D-printed and assembled in-house. Open-source microcontrollers (i.e., Arduino) were used to control individual hardware modules with Marlin as a firmware written in C++ and the user interface written in Python for serial communication with microcontrollers.

Benchmarking Organic Micropollutants in Wastewater, Recycled Water and Drinking Water with In Vitro Bioassays

Xiaowei Zhang

Thousands of organic micropollutants and their transformation products occur in water. Although often present at low concentrations, individual compounds contribute to mixture effects. Cell-based bioassays that target health-relevant biological endpoints may therefore complement chemical analysis for water quality assessment. The objective of this study was to evaluate cell-based bioassays for their suitability to benchmark water quality and to assess efficacy of water treatment processes. The selected bioassays cover relevant steps in the toxicity pathways including induction of xenobiotic metabolism, specific and reactive modes of toxic action, activation of adaptive stress response pathways and system responses. Twenty laboratories applied 103 unique in vitro bioassays to a common set of 10 water samples collected in Australia, including wastewater treatment plant effluent, two types of recycled water (reverse osmosis and ozonation/activated carbon filtration), stormwater, surface water, and drinking water. Sixty-five bioassays (63%) showed positive results in at least one sample, typically in wastewater treatment plant effluent, and only five (5%) were positive in the control (ultrapure water). Each water type had a characteristic bioanalytical profile with particular groups of toxicity pathways either consistently responsive or not responsive across test systems. The most responsive health-relevant endpoints were related to xenobiotic metabolism (pregnane X and aryl hydrocarbon receptors), hormone-mediated modes of action (mainly related to the estrogen, glucocorticoid, and antiandrogen activities), reactive modes of action (genotoxicity) and adaptive stress response pathway (oxidative stress response). This study has demonstrated that selected cell-based bioassays are suitable to benchmark water quality and it is recommended to use a purpose-tailored panel of bioassays for routine monitoring.

American Chemical Society2014

Design approach for the improvement of the economic and environmental performances of potable water supply

François Vince

Triggered by the Kyoto Protocol and by the aggravation of freshwater scarcity, environmental impacts should soon become key decision criteria for the planning of potable water supply projects, especially when advanced systems such as seawater desalination are at stake. In order to foster the transition of the water industry towards sustainable practices, the present work proposes an integrated design approach dedicated to potable water supply that targets both economic and environmental objectives. At first, the current industrial practices (Chapter I) and the technical characteristics of potable water supply systems (Chapter II) are analyzed via the modeling of the process units used for potable water supply: pumping systems, conventional water treatment processes (e.g. clarification, filtration, disinfection) and advanced water treatment processes (e.g. membrane processes, thermal processes). Using the ISO 14040 standardized Life Cycle Assessment (LCA) method, Chapter III presents the development of a performance indicator that assesses the environmental impacts generated through all stages of the life cycle of potable water supply (i.e. from "cradle to grave"): from the construction of the potable water treatment plant, to its operation and decommissioning. This LCA-based indicator provides a holistic overview of all potential environmental impacts (e.g. green house gases (GHG) emissions, impacts on ecosystems and on human health). It allows to stress out the impact sources and the penalizing steps within potable water supply (Chapter IV): The production of electricity and chemicals required by the potable water treatment plant is highlighted to generate respectively 75% and 15% of the total impacts generated during the life cycle of potable water supply. Different potable water supply scenarios (e.g. potable water supply from ground water, from surface water, seawater desalination, water import from distant water resources) are benchmarked, in order to identify the best solutions as a function of the local context (e.g. type of electricity supply, topographic conditions, feed water quality). Based on the results of the environmental assessment, Chapter V proposes measures for the improvement of the industrial practices of the water sector, targeting energy and chemicals management, electricity sourcing and effluent disposal. Within the same perspective, Chapter VI details an optimization method for the design of the reverse osmosis (RO) membrane process, i.e. the key treatment process for desalination and wastewater reclamation. This method systematically synthesizes RO process configurations and evaluates their performances on economical (total annualized costs), technical (energy requirement, water conversion rate) and environmental criteria (GHG emissions). Evolutionary algorithms are then used to optimize the design of these configurations (process layout and operating conditions) and to identify those featuring the best trade-offs between economical costs and environmental impacts. As case study, the optimization method is applied on a brackish water reverse osmosis (BWRO) desalination project. The characteristics of the optimal BWRO process configurations are calculated as a function of the project constraints (e.g. economical and technical settings, minimum potable water quality), in order to illustrate how this method may support process engineers for the design of desalination plants.

EPFL2008

Recyclage des eaux usées en irrigation

Yeli Mariam Sou

The present study takes place in Ouagadougou (Burkina Faso), a typical Sub-Saharan city where water shortage and food crisis necessitate wastewater reuse in urban agriculture. Different issues linked to this practice were studied in two components separated in two experimental sites. The first part treats about agronomic and sanitary aspects and take place in the 2iE (International Institute for Water and Environmental Engineering) experimental site of Ouagadougou. The second component was emphasised on industrial effluents impacts on irrigated soil quality and takes place in Kossodo, an industrial suburb of Ouagadougou. The 2iE site is supply with treated wastewater from domestic origin. The effluent was used to realise the following objectives: (1) managed wastewater fertilizers to fit the plant needs; (2) assess different crops sanitary quality and the effectiveness of methods used for this assessment. Three treatments were tested: (i) irrigation with the treated wastewater (ii) irrigation with the treated wastewater and fertilizers management, adjusted to plant needs and (iii) conventional treatment i.e. fresh water irrigation and total doses of commercial fertilizers. Each treatment was tested on three different crops: lettuce (Lactuca sativa), carrot (Daucus carota) and eggplant (Solanum melongena). The first objective was achieved by comparing crops yields (on basis of wet and dry matter) obtained with the different treatments. The three crops, irrigated with only wastewater, led to yield reduction between the first and the second year. At the contrary, the adjusted treatment showed higher yield for lettuce, with comparable values to conventional treatment yield. However, the effects on carrot and eggplant did not demonstrate better results; indicating that further investigations must be completed for crops with longer growing periods. Most likely, the fertilizers management should better correspond to the crop period demand. The sanitary assessment (second objective) was performed by measuring the heavy metals concentration (Cd, Cu, Pb, Ni, Cr) and the faecal levels (faecal coliform, Escherichia coli and four pathogens among which Salmonella typhimirium and Vibrio cholerae). Chromocult Coliform Agar medium was used for simultaneous detection of faecal coliform and Escherichia coli whereas pathogens were analysed by Polymerase Chain Reaction (PCR). From methodological point of view, faecal coliform were not sufficiently discriminatory in the case of this study since they were naturally present in the soil as well as in the fresh water. At the contrary, Escherichia coli were better linked to wastewater contamination. However, some "false positive" results were detected meaning that each presumptive colony of Escherichia coli must be confirmed. Nevertheless, microbial quality assessment indicated no Escherichia coli on edible parts of carrot or eggplant irrigated with wastewater. Carrots were expected to have the poorest microbiological quality. Therefore carrot root bacterial quality must be further examined. Results on eggplant are not surprising as these vegetables are somewhat far from the ground and they receive significant solar radiation which acts like a disinfectant. Lettuce from wastewater treatment plots, however, exhibited presence of Escherichia coli, meaning that lettuce is likely to be contaminated by pathogens. However, no pathogens were detected neither on crops irrigated with fresh water nor on those irrigated with wastewater. Heavy metals results demonstrated that the crop's consumption did not involve higher risk for consumers when the vegetables were irrigated with domestic wastewater, in comparison with fresh water irrigation. Kossodo treatment plant receives mainly industrial wastewater, which are characterized by high levels of sodium and bicarbonates. The treated wastewater supplies a market garden in which space has been defined for the study of this second component. The main objective was to assess the impact of treated wastewater on soil quality during two years of irrigation. Early in the second year, the presence of black spots, suspected to be black alkali (dissolved organic matter), appeared at the surface of plots irrigated with treated wastewater. These indications led to put forward the hypothesis of alkalinisation/sodisation on soil receiving wastewater. The methodological approach used was based on in situ measurement of infiltration at the end of the second year of irrigation, supplemented by shrinkage curve modelling and chemical analysis. The hypothesis of an alkalinization/sodisation and dissolved organic matter (black alkali) presence in plots irrigated with treated wastewater were validated by the chemical analysis. Shrinkage curve modelling showed a reduction of the structural porosity on wastewater irrigated plots. The reduction was more marked in the subsurface horizons than in surface, which is probably caused by dispersed clay leached in depth. These structural changes were confirmed in situ by an infiltration capacity greatly reduced on plots irrigated with treated wastewater, compared to control plots (irrigated with fresh water) where the infiltration capacity is higher and comparable to that of non-irrigated soil. The study confirmed the fertilizing value of domestic wastewater while emphasizing the need to concentrate future investigations on sustainable effluent/fertilizers management. The sanitary assessment with different crops regarding their respective distances to the soil was very useful since it showed the strong interaction of soil microbial community on the crop microbial quality. It also revealed the importance to determine a faecal indicator for the crop, specifically related to wastewater contamination. Soil quality assessment reiterated the necessity to verify wastewater quality before its reuse in agriculture. In the case of this study, the sodic and alkaline properties of the industrial effluents make them definitively unfit for irrigation.

EPFL2009