System and method for single cell phenotypical profiling and deterministic nanoliter-droplet encapsulation and deterministic droplet consortia assemblies

The present invention concerns a system for phenotypical profiling of at least one object and deterministic nanoliter-droplet encapsulation, comprising sample supplying means, buffer supplying means; a microfluidic chip comprising an encapsulation area or structure in which the object is encapsulated with a quantity of the reaction buffer by the droplet; detection means configured to detect the passage of the object through the first imaging chamber; at least one valve configured to stop the flow of the sample buffer when the detection means detect passage of the object through the first imaging chamber; phenotypical assessing means configured to assess the phenotype of the object when the flow of the sample buffer is stopped by the valve and the object is at an object stopping site; a droplet deposition means configured to deposit the droplet in a well or in a well of a multi-well plate and comprising an outlet capillary.