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Design of an artificial phage-display library based on a new scaffold improved for average stability of the randomized proteins

Publications associées (56)

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High-sensitive protein analysis by FESI-CEMALDI-MS

Hubert Girault, Mohammad Reza Pourhaghighi

Capillary zone electrophoresis (CZE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) are two techniques highly suitable for the separation and detection of intact proteins. Herein, based on the use of a recently introduced iont ...

Wiley-Blackwell2011

Solute energy based REMD

Molecular dynamics (MD) simulations have increasingly contributed to the understanding of biomolecular processes, allowing for predictions of thermodynamic and structural properties. Unfortunately, the holy grail of protein structure prediction was soon fo ...

EPFL2008

Photodissociation of Alkyl Iodides in Helium Nanodroplets III: Recombination

Marcel Drabbels, Andreas Braun

The recombination of fragments resulting from the photodissociation of (fluorinated) alkyl iodides in helium nanodroplets at a wavelength of 266 nm has been investigated by means of ion imaging techniques. It is found that in the case of CH3I an appreciabl ...

2007

Using triplet-triplet energy transfer to measure conformational dynamics in polypeptide chains

Beat Fierz

Intrachain diffusion processes play an important role in protein folding and function. In this chapter we discuss the application of triplet-triplet energy transfer to directly measure rate constants for intrachain contact formation in polypeptide chains. ...

Humana Press2006

Engineering of the human O6-alkylguanine DNA alkyltransferase

Alexandre Juillerat

Recently, a new method for the specific covalent labeling of fusion proteins in vitro and in living cells has been developed. This method is based on the unusual mechanism of a DNA repair protein: the O6-alkylguanine DNA alkyltransferase (AGT). In addition ...

EPFL2005

The central domain of the matrix protein of HIV-1: influence on protein structure and virus infectivity

Oliver Hantschel

The central region of the matrix protein p17 of HIV-1 is known to be essential during virus assembly. We substituted alanines for amino acid triplets in this region of p17 (amino acid residues 47 to 55: NPG LLE TSE). Introduction of the respective mutation ...

Walter de Gruyter2004

Exploring the Capabilities of Nucleic Acid Polymerases by Use of Directed Evolution

Kai Johnsson, Petra Tafelmeyer

A review. Directed evolution consists of repetitive cycles of random mutagenesis of the protein/peptide sequence followed by screening or selection of candidates with desired properties. Many different approaches are used to introduce mutations into a gene ...

Wiley2004

Metal ion assisted folding and supramolecular organization of a De Novo designed metalloprotein

Harm-Anton Klok

This paper describes the supramolecular organization of a novel de novo designed metalloprotein, which consists of two N-terminal terpyridine modified coiled-coil protein folding motif sequences held together by an iron(II) ion. The self-assembly of the me ...

2004

Protein design and folding: Template trapping of self-assembled helical bundles

An experimental system is described, permitting a detailed and systematic analysis of the factors governing self-assembly of amphipathic helices, e.g. to a four-helical bundle, a subject of major relevance for tertiary structure formation. protein folding ...

2001

Evolutionary approaches to study cytochrome c peroxidase

Kai Johnsson, Petra Tafelmeyer

A review with 15 refs. Directed mol. evolution of enzymes and proteins has emerged as an extremely powerful method to create proteins with novel properties, both for practical applications as well as for mechanistic studies. To demonstrate the underlying p ...

2001