Systematic benchmarking of single-cell ATAC-sequencing protocols
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Chromatin-immunoprecipitation and sequencing (ChIP-seq) is a rapidly maturing technology that draws on the power of high-throughput short-read sequencing to decipher chromatin states with unprecedented precision and breadth. Although some aspects of the ex ...
Fluorescent 2'-deoxynucleotides containing a protecting group at the 3'-O-position are reversible terminators enabling array-based DNA sequencing by synthesis (SBS) approaches. Herein, we describe the synthesis of a new family of 3'-OH unprotected cleavabl ...
Advances in sequencing technology are yielding DNA sequence data at an alarming rate – a rate reminiscent of Moore's law. Biologists' abilities to analyze this data, however, have not kept pace. On the other hand, the discrete and mechanical nature of the ...
Landscape genomics is an emerging research field that bridges genetics and genomics to geo-environmental resources analysis. It aims to study genome/environment interaction to discover the genetic basis of adaptation by processing of many simultaneous DNA- ...
Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture2010
Aim: In molecular microbial ecology, pyrosequencing is gradually supplanting classical fingerprinting techniques such as terminal-restriction fragment length polymorphism (T-RFLP) combined with cloning-sequencing for enhanced characterization of microbiome ...