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Protocol for live imaging of intracellular nanoscale structures using atomic force microscopy with nanoneedle probes

Publications associées (37)

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Un hôpital psychiatrique au XXIe siècle (Région Parisienne, Stains, F)

Sophie Lufkin

L'épidémie de dépression qui affecte lourdement nos sociétés occidentales est le point de départ d'une démocratisation et d'une dédramatisation de la psychiatrie. Face à l'évolution des pratiques médicales et sociales, le caractère de l'hôpital psychiatriq ...

2005

Covalent labeling and chemical induced dimerization of fusion proteins

A new method was developed for the specific and covalent labeling of fusion proteins with small organic probes in vitro and in vivo. The method is based on the construction of fusion proteins of the human O6-alkylguanine-DNA alkyltransferase (hAGT) with a ...

EPFL2005

A new technology for the specific and covalent labelling of fusion proteins with small organic molecules in vivo and in vitro has recently been developed in our laboratory. The method is based on the genetically encoded fusion of a target protein to O6-alk ...

EPFL2005

Imaging cells and tissues with refractive index radiology

Giorgio Margaritondo, Amela Groso, Yong Yang

Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissue ...

2004

Specific Labeling of Cell Surface Proteins with Chemically Diverse Compounds

Horst Vogel, Kai Johnsson, Horst Pick, Nathalie George

The specific and covalent labeling of fusion proteins with synthetic mols. opens up new ways to study protein function in the living cell. Here the authors present a novel method that allows for the specific and exclusive extracellular labeling of proteins ...

2004

Many fundamental processes that govern the function of biological cells occur on or immediately under the cell membrane. Fluorescence marking of specific sites has had a large impact in the recognition of these processes. In addition, observation in an app ...

EPFL2000

The clustering of telomeres and colocalization with Rap1, Sir3, and Sir4 proteins in wild-type Saccharomyces cerevisiae

Thierry Laroche

We have developed a novel technique for combined immunofluorescence/in situ hybridization on fixed budding yeast cells that maintains the three-dimensional structure of the nucleus as monitored by focal sections of cells labeled with fluorescent probes and ...

1996