Publication
Structure-function analysis of the cyclic β-1,2-glucan synthase from Agrobacterium tumefaciens
Publications associées (36)
Microsecond Time-Resolved Cryo-Electron Microscopy
Recently, single-particle cryo-electron microscopy emerged as a technique capable of determining protein structures at near-atomic resolution and resolving protein dynamics with a temporal resolution ranging from second to milliseconds. This thesis describ ...
EPFL2024Mechanism ofcyclic beta-glucan export by ABC transporter Cgt of Brucella
Henning Paul-Julius Stahlberg, Dongchun Ni, Na Wu
Polysaccharides play critical roles in bacteria, including the formation of protective capsules and biofilms and establishing specific host cell interactions. Their transport across membranes is often mediated by ATP-binding cassette (ABC) transporters, wh ...
NATURE PORTFOLIO2022New Methods for the Integrative Dynamic Modeling of Biomolecular Structures
The true understanding of most cellular functions is only really achievable through the structural determination of their underlying macromolecular assemblies. However, their size, large number of individual components and metastable states make their eluc ...
EPFL2021Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly
Georg Fantner, Georgios Hatzopoulos, Tatiana Favez, Oliver Hantschel, Virginie Hamel, Niccolo Banterle, Santiago Harald Andany
Centrioles are evolutionarily conserved multi-protein organelles essential for forming cilia and centrosomes. Centriole biogenesis begins with self-assembly of SAS-6 proteins into 9-fold symmetrical ring polymers, which then stack into a cartwheel that sca ...
NATURE RESEARCH2021High-resolution cryo-EM structure of urease from the pathogen Yersinia enterocolitica
Henning Paul-Julius Stahlberg, Jasenko Zivanov
Urease converts urea into ammonia and carbon dioxide and makes urea available as a nitrogen source for all forms of life except animals. In human bacterial pathogens, ureases also aid in the invasion of acidic environments such as the stomach by raising th ...
2020Will Cryo-Electron Microscopy Shift the Current Paradigm in Protein Structure Prediction?
Matteo Dal Peraro, Luciano Andres Abriata
Protein dynamics is undoubtedly a pervasive ingredient in all biological functions. However, structural biology has been strongly driven by a static-centered view of protein architecture. We argue that the recent advances of cryo-electron microscopy (EM) h ...
AMER CHEMICAL SOC20203D Poses Recovery in Single-Particle Cryo-EM from Learned Pairwise Projection Distances
Single-particle cryo-electron microscopy (cryo-EM) is a technology that allows the observation and the high-resolution 3D structure determination of biomolecules. In this project, the goal is to estimate the angles at which we imaged the 2D projections fro ...
2020Cryo-EM structure of phosphodiesterase 6 reveals insights into the allosteric regulation of type I phosphodiesterases
Henning Paul-Julius Stahlberg, Andreas Engel
Cyclic nucleotide phosphodiesterases (PDEs) work in conjunction with adenylate/guanylate cyclases to regulate the key second messengers of G protein-coupled receptor signaling. Previous attempts to determine the full-length structure of PDE family members ...
American Association for the Advancement of Science (AAAS)2019Microfluidic protein isolation and sample preparation for high-resolution cryo-EM
Henning Paul-Julius Stahlberg, Inayathulla Mohammed
High-resolution structural information is essential to understand protein function. Protein-structure determination needs a considerable amount of protein, which can be challenging to produce, often involving harsh and lengthy procedures. In contrast, the ...
Proceedings of the National Academy of Sciences2019Structural and functional analysis of host attachment proteins from contractile injection systems
Bacteriophages of the myoviridae family, R-type pyocin, Photorhabus virulence cassette (PVC), Serratia antifeeding prophage (Afp) and type 6 secretion system (T6SS) form a class of contractile injection systems that share common structural and functional c ...
EPFL2018