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Advancing Cell-Free Synthetic Biology: From sustained PURE protein synthesis to an isothermal one-pot DNA replication system

Publications associées (46)

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Microfabrication of free-standing micro-solid oxide fuel cells on Foturan® and silicon substrates

Nico de Rooij, Danick Briand, Paul Muralt, Jérôme Courbat, Yan Yan

Due to their high specific energy and energy density, micro-solid oxide fuel cells1,2 (µ-SOFCs) are seen as one of the technologies that could help satisfying the continuously increasing demand for electric energy in portable electronic devices. The µ-SOFC ...

2011

Dual-wavelength Digital Holography for quantification of cell volume and integral refractive index (RI)

Pierre Magistretti, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Daniel Boss

Quantitative Phase Imaging techniques such as DHM have emerged recently in life sciences and can be aimed at monitoring and quantifying non-invasively dynamic cellular processes modifying cell morphology and/or content. Concretely, the DHM phase signal dep ...

SPIE2011

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The study of membranes is at a turning point. New theories about membrane structure and function have recently been proposed, however, new technologies, combining chemical, physical, and biochemical approaches are necessary to test these hypotheses. In par ...

2011

Preparation of nucleic acid templates for solid phase amplification

Gerardo Turcatti

The invention relates to a method of preparing template constructs for solid-phase nucleic acid amplification and to use of the templates in methods of solid-phase nucleic acid amplification. The method involves carrying out two ligation reactions: (a) a l ...

2010

Tissue engineering of the bladder

Catharina Adelöw

Stem cell use in bladder tissue engineering is a recently addressed area of investigation that has generated excitement as a novel way to restore and regenerate lost or damaged urinary bladder tissue. The remodelling of smooth muscle plays a significant ro ...

EPFL2009

Studies on scaffold design for tissue engineering applications

The aim of tissue engineering is to regenerate tissue for the purpose of repairing or replacing diseased or injured tissue. Therefore, cells that are able to proliferate are seeded on scaffolds that provide mechanical stability and direct the three-dimensi ...

EPFL2009

Miniaturized bioanalytics to probe the function of membrane proteins

Pedro Pascoal

G-protein coupled receptors (GPCRs) are the most abundant class of proteins in the cell body. Such receptors are of major interest as potential therapeutic targets. Downscaling and parallelization of bioanalytics opens novel routes to rapidly screen and id ...

EPFL2008

Language Evolution as a Darwinian Process: Computational Studies

Frédéric Kaplan

This paper presents computational experiments that illustrate how one can precisely conceptualize language evolution as a Darwinian process. We show that there is potentially a wide diversity of replicating units and replication mechanisms involved in lang ...

2007

Under the influence of phi and psi

A review. Topics include pseudo-prolines (YPro) as a solubilizing, structure-disrupting protection technique in peptide synthesis and the concept of Template-Assembled-Synthetic Proteins (TASP). [on SciFinder (R)] ...

2005

Specification of Replication Techniques, Semi-Passive Replication, and Lazy consensus*

André Schiper, Xavier Défago

This paper brings the following three main contributions: a hierarchy of specifications for replication techniques, semi-passive replication, and Lazy Consensus. Based on the definition of the Generic Replication problem, we difine two families of replicat ...

2002