Advancing Cell-Free Synthetic Biology: From sustained PURE protein synthesis to an isothermal one-pot DNA replication system
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The oligomerization of activated D- and L- and racemic guanosine-5'-phosphoro-2-methylimidazole on short templates contg. D- and L-deoxycytidylate has been studied. Results obtained with D-oligo(dC)s as templates are similar to those previously reported fo ...
A symposium report. The concept of template assembled synthetic proteins (TASP) has been evaluated for the locked-in fold derived from the DNA-binding bba zinc finger motif based on the consensus sequence Xaa3-Cys-Xaa2-4-Cys-Xaa12-His-Xaa3-4-His-Xaa4 to mi ...
1999
The structural prototype of a new generation of regioselectively addressable functionalized templates (RAFTs) for use in protein de novo design has been synthesized and crystallized. The structure of the aromatically substituted cyclodecapeptide was determ ...
2001
The sequential condensation of peptide loops onto selectively addressable templates immobilised on solid supports allows the rapid and efficient synthesis of protein mimetics applying the TASP (Template Assembled Synthetic Protein) concept. As an example o ...
1997
A review with 38 refs. The ultimate goal in protein de novo design is the construction of artificial proteins exhibiting tailor-made structural and functional properties. To create native-like macromols. in copying nature's way had proven to be difficult b ...
1996
A symposium report on synthetic strategies which provide a synthetic entry to a new generation of template assembled synthetic proteins (TASP). [on SciFinder (R)] ...
1998
A review with twenty-nine refs. on the authors' current research. Protein design and mimicry combines elements of synthetic org. chem. with structural and functional aspects of biol. relevance in a unique way. Due to progress in this interdisciplinary rese ...
2000
A symposium report. We report on the synthesis of TASP (template assembled synthetic proteins) mols. able to bind to neurotensin Y and angiotensin II receptors, in which a-cyclodextrin is used as template. [on SciFinder (R)] ...
1999
A review with 43 refs. on strategies for the synthesis of protein mimetics and artificial proteins. The ultimate goal in protein de novo design is the construction of artificial proteins inhibiting tailor-made structural and functional properties. To creat ...
1996
A review, with 33 refs. The authors review here the template-assembled synthetic proteins concept and some of its extensions in peptide mimicry and supramol. chem. [on SciFinder (R)] ...
