Lock-in amplified differential phase contrast

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Multicore fiber endoscope for phase imaging based on intensity recording using deep neural networks

Demetri Psaltis, Christophe Moser, Navid Borhani, Eirini Kakkava

The present invention is related to an endoscopic system for phase imaging, comprising a multicore waveguide (080), an optical system comprising at least one first light source (002), for illuminating the sample to be examined, a first camera (010) that is ...

2022

Advanced Techniques in Optical Diffraction Tomography

Ahmed Bassam Sayed Ayoub Mohamed Emam

In this thesis, we study the 3 challenges described above. First, we study different reconstruction techniques and assess the fidelity of each reconstruction results by means of structured illumination and phase conjugation. By reconstructing the 3D refrac ...

EPFL2022

Magnetic force microscopy contrast formation and field sensitivity

Marcos Penedo Garcia, Johannes Schwenk

The magnetic force microscope (MFM) is an established experimental tool for imaging stray fields with high spatial resolution and sensitivity. The MFM contrast can however contain contributions from the sample topography, variations in the surface Kelvin p ...

2022

Optical microscopy gets down to angstroms

Suliana Manley, Giorgio Tortarolo

Super-resolution microscopy at angstrom precision could pave the way to optical structural biology in cells. ...

NATURE PORTFOLIO2022

In-operando dynamic visualization of flow through porous preforms based on X-ray phase contrast imaging

Véronique Michaud, Baris Çaglar, Helena Luisa Teixido Pedarros

Direct visualization is often sought to elucidate flow patterns and validate models to predict the filling kinetics during processes whereby a liquid resin infiltrates a textile porous preform. Here, X-ray phase contrast interferometry is evaluated to imag ...

2021

Robust Phase Unwrapping via Deep Image Prior for Quantitative Phase Imaging

Michaël Unser, Matthias Lütolf, Nathalie Brandenberg, Thanh-An Michel Pham, Fangshu Yang

Quantitative phase imaging (QPI) is an emerging label-free technique that produces images containing morphological and dynamical information without contrast agents. Unfortunately, the phase is wrapped in most imaging system. Phase unwrapping is the comput ...

2021

3D reconstruction of weakly scattering objects from 2D intensity-only measurements using the Wolf transform

Demetri Psaltis, Joowon Lim, Elizabeth Elena Antoine, Ahmed Ayoub

A new approach to optical diffraction tomography (ODT) based on intensity measurements is presented. By applying the Wolf transform directly to intensity measurements, we observed unexpected behavior in the 3D reconstruction of the sample. Such a reconstru ...

2021

Polarization-sensitive optics diffraction tomography

Demetri Psaltis, Joowon Lim, Elizabeth Elena Antoine, Amirhossein Saba Shirvan, Ahmed Ayoub

Polarization of light has been widely used as a contrast mechanism in two-dimensional (2D) microscopy and also in some three-dimensional (3D) imaging modalities. In this paper, we report the 3D tomographic reconstruction of the refractive index (RI) tensor ...

2021

Phase calibration of a basic bright-field microscope for 3D metrology of transparent samples at the nanoscale

Martinus Gijs, Daniel Migliozzi, Bingying Zhao

Optical retrieval of the structure of transparent objects at the nano-scale requires adapted methods capable of probing their interaction with suitable light. Scattering events, for instance, depend on the content and the arrangement of the medium encounte ...

SPIE-INT SOC OPTICAL ENGINEERING2021

Quantitative Microstructural Imaging for Clinical Use

Gian Franco Piredda

Today, Magnetic Resonance Imaging (MRI) is a well-established medical imaging modality thanks to its excellent soft tissue contrast. Conventional MR image contrasts can be weighted towards one or more physical properties that discriminate different tissues ...

EPFL2021

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NATURE PORTFOLIO2022