RETENTION BEHAVIOR OF A TEMPLATE-ASSEMBLED SYNTHETIC PROTEIN AND ITS AMPHIPHILIC BUILDING-BLOCKS ON REVERSED-PHASE COLUMNS
Graph Chatbot
Chattez avec Graph Search
Posez n’importe quelle question sur les cours, conférences, exercices, recherches, actualités, etc. de l’EPFL ou essayez les exemples de questions ci-dessous.
AVERTISSEMENT : Le chatbot Graph n'est pas programmé pour fournir des réponses explicites ou catégoriques à vos questions. Il transforme plutôt vos questions en demandes API qui sont distribuées aux différents services informatiques officiellement administrés par l'EPFL. Son but est uniquement de collecter et de recommander des références pertinentes à des contenus que vous pouvez explorer pour vous aider à répondre à vos questions.
Proteolytic activation, oligomerization, and membrane insertion are three steps that precede channel formation by the bacterial toxin aerolysin. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and hydrogen-deuterium exc ...
For a comparison with the corresponding alpha- and beta-hexapeptides H-(Val-Ala-Leu)2-OH (A) and H-(beta-HVal-beta-HAla-beta-HLeu)2-OH (B), the corresponding gamma-hexapeptide (I), built from the homochirally similar (S)-4-aminobutanoate, (R)-4-amino-5-met ...
A review with 37 refs. In the present review, various attempts to apply synthetic tools for inducing native-like structural features in peptides in order to bypass the folding problem are described. Besides well-established methods for the nucleation and s ...
A serotonin 5-HT3 receptor was functionally expressed to high levels and on a large scale in mammalian cells with the Semliki Forest virus system. Conditions were optimized to maximize detergent solubilization of the receptor, while preserving ligand bindi ...
A pore-forming peptide is implicated in the potent cytolytic activity of pathogenic Entamoeba histolytica. Using NH2-terminal sequence information of this peptide, the corresponding cDNA was isolated. The cDNA-deduced amino acid sequence revealed a putativ ...
This paper reports the synthesis of rationally designed polypeptides that catalyze the decarboxylation of oxaloacetate via an imine intermediate. The authors det. the secondary structures of the polypeptides by two-dimensional NMR spectroscopy. The authors ...
Enantiomerically pure beta-amino acid derivs. with the side-chains of Ala, Val, and Leu in the 2- or 3-position (β2- and β3-amino acids, resp.), as well as with substituents in both the 2- and 3-positions (β2,3-amino acids, of like-configuration) were prep ...
The purification to homogeneity of an active soluble 25 kDa fragment of CD23, produced in insect cells using the baculovirus expression system, is described. Peptide mapping and analysis by Edman degradation and mass spectrometry permitted partial characte ...
Aerolysin is a channel-forming protein secreted as a protoxin by Aeromonas hydrophila. Analytical centrifugation measurements showed that proaerolysin is a dimer in solution, and this was confirmed by chemical cross-linking with dimethyl suberimidate. Diss ...
beta-Hexapeptides and a beta-dodecapeptide with sequences contg. two different types of beta-amino acids, aliph. proteinaceous side-chains in 2- or 3-position, were studied with CD and NMR measurements. With one exception, the secondary structures formed b ...
