Contrasting the excited-state dynamics of the photoactive yellow protein chromophore: Protein versus solvent environments
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A general approach for the sequential labeling of fusion proteins of O6-alkylguanine-DNA alkyltransferase (AGT) with different fluorophores in mammalian cells is presented. AGT fusion proteins with different localizations in the cell can be labeled specifi ...
Proteins have a complex free-energy landscape because of their rich topol. and the nature of their nonbonded interaction potential. This has important consequences because the roughness of the landscape affects the ease with which a chain folds and also de ...
Time-integrated fluorescence expts. on native bacteriorhodopsin and on its non-isomerizing form bR5.12 are reported. The exptl. set-up was designed such as to observe emission exclusively from the excited state intermediate I-460. We obtain the first syste ...
Steady-state and picosecond (ps) fluorescence studies of wild-type bacteriorhodopsin (wt-bR) and of a nonisomerizing analog locked in the all-trans configuration have been performed. Extending earlier work done by femtosecond absorption spectroscopy, we ob ...
A symposium report. The thermal stability of beta-peptides is discussed and a mechanism for unfolding and folding is proposed. beta-Peptide helixes of only seven residues are stable up to 80 Deg and above in CD3OH. ...
The spectrally and temporally resolved fluorescence properties of native bacteriorhodopsin (bR) and bR reconstituted with a nonisomerizing analog of the retinal Schiff base (bR5.12) are examd. The first attempt to exptl. monitor the excited state relaxatio ...
A review with 6 refs. of the authors' work in developing a biophys. technique to investigate the structure, function and dynamics of membrane receptors. A fluorescence-based approach has been developed and applied to the prototypic G protein-coupled recept ...
