The importance of ammonia in mammalian cell culture
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We have purified a 100 kDa protein, resolved in a Southwestern binding screen of total nuclear proteins from Hela cells with double-stranded human telomeric probe. A polyclonal antiserum raised by this protein recognizes purified nucleolin and stains nucle ...
Amino acid transport across cellular membranes is mediated by multiple transporters with overlapping specificities. We recently have identified the vertebrate proteins which mediate Na+-independent exchange of large neutral amino acids corresponding to tra ...
Members of the newly discovered glycoprotein-associated amino acid transporter family (gpaAT-family) share a similar primary structure with >40% identity, a predicted 12-transmembrane segment topology and the requirement for association with a glycoprot ...
Batch cultures of a mouse/mouse hybridoma cell line secreting a dimeric IgA were performed in combination with a membrane process for the continuous removal of ammonia from the culture medium. The latter involves hydrophobic porous membranes with an assocd ...
Anti- V.cholerae monoclonal IgA antibodies have been produced using a hybridoma cell line (Zac3) grown in RPMI medium contg. 10% Fetal calf serum (FCS) and a completely defined protein- free medium. The latter has been optimized with respect to amino acid, ...
In order to investigate cell-specific differences in the response of in vitro models to environmental toxicants, we compared the capacity of nine polycyclic aromatic hydrocarbons (PAHs) to induce cytochrome P4501A (CYP1A) in primary rainbow trout (Oncorhyn ...
Society of Environmental Toxicology and Chemistry Press2001
Comprehensive and cGMP suitable bioprocess monitoring is still a challenge in microbial fermn. and cell culture process development. This paper discusses the exploitation of in-situ mid-IR spectroscopy in combination with partial least-squares anal. to mon ...
Methodology was developed for quantifying the photocytotoxicity of fluoranthene to a gill cell line from rainbow trout for future use in screening polycyclic aromatic hydrocarbons for their relative photocytotoxicity to fish. Solubilization in a modified c ...
A balanced supplementation method was applied to develop a serum and protein- free medium supporting hybridoma cell batch culture. The aim was to improve systematically the initial formulation of the medium to prevent limitations due to unbalanced concns. ...
GAD67, the larger isoform of the γ-aminobutyric acid-synthesizing enzyme glutamic acid decarboxylase, is a hydrophilic soluble molecule, postulated to localize at nerve terminals and membrane compartments by heterodimerization with the smaller membrane-anc ...
American Society for Biochemistry and Molecular Biology1999
