Identification and control of oxidative metabolism in Saccharomyces cerevisiae during transient growth using calorimetric measurements
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The calorimetric response of the yeast Kluyveromyes fragilis was investigated for growth in continuous culture where N limitation was imposed on a C-limited culture. Calorimetric measurements were combined with off gas anal., measurements of biomass, subst ...
Using a specific 13C NMR localization method, 13C label incorporation into the glycogen C1 resonance was measured while infusing [1- 13C]glucose in intact rats. The maximal concentration of [1-13C]glycogen was 5.1 ± 0.6 μmol g-1 (mean ± SE, n = 8). During ...
Aerobic chemostat cultures of Saccharomyces cerevisiae were performed under carbon-, nitrogen-, and dual carbon- and nitrogen-limiting conditions. The glucose concn. was kept const., whereas the ammonium concn. was varied among different expts. and differe ...
A tight coupling exists between neuronal activity and energy metabolism. Over a century ago Roy and Sherrington postulated that “the brain possesses intrinsic mechanisms by which its vascular supply can be varied locally in correspondence with local variat ...
The alpha-(1-->3)- and alpha-(1-->4)-linked C-disaccharides derived from D-glucose and L-mannose have been obtained with high stereoselectivity via the addition of 2,3,4,6-tetra-O-acetyl-glucopyranosyl radical to (1S,4R,5R,6R)-6-endo-chloro-3-methylidene-5 ...
Glucose is the main fuel for energy metabolism in the normal human brain. It is generally assumed that glucose transport into the brain is not rate-limiting for metabolism. Since brain glucose concentrations cannot be determined directly by radiotracer tec ...
Heat produced by hybridoma suspension cultures was continuously monitored in a 2-L heat-flux calorimeter. Heat measurements reflected changes in the metabolic state of the culture under different aeration conditions. A highly anaerobic metab. was indicated ...
Adenosine promotes a concentration-dependent hydrolysis of 3H-glycogen newly synthesized from 3H-glucose by mouse cerebral cortical slices. The EC50 for this effect is 7 microM. Theophylline antagonizes the glycogenolysis induced by adenosine with an EC50 ...
The IC-compatible lift-off technique is applied for patterning enzymatic membranes on wafer level. They are used to complete planar three-electrode microelectrochemical cells. These final devices, based on amperometric H2O2 detection, are tested for the de ...
