The effect of cell line, transfection procedure and reactor conditions on the glycosylation of recombinant human anti-rhesus D IGG1

An anti-Rhesus D antibody was produced in various cell-lines and cell-types which were either stable or transient transfected and grown under varying culture conditions. Glycosylation anal. of the IgG1 was carried out mainly by HPAEC methods. The antibody was N-glycosylated only at the conserved glycosylation site at Asn-297 carrying complex type N-glycans as usually present on IgG. The core structure was entirely fucosylated and did not carry intersecting G1cNAc for all antibodies analyzed. Prodn. in SP2/0, CHO and HEK-293 cells resulted in significantly different oligosaccharide patterns. The influence of the cultivation conditions on the glycosylation was usually less than the influence of cell-type. The glycosylation pattern of IgG produced in transient transfected CHO cells was different than in stable transfected CHO cells but the differences obsd. might be also due to different culture conditions. Further factors influencing the glycosylation were medium compn. and the type of bioreactor used for the cultivation. [on SciFinder (R)]