The secondary structure of bacteriorhodopsin determined by Raman and circular dichroism spectroscopy
Graph Chatbot
Chattez avec Graph Search
Posez n’importe quelle question sur les cours, conférences, exercices, recherches, actualités, etc. de l’EPFL ou essayez les exemples de questions ci-dessous.
AVERTISSEMENT : Le chatbot Graph n'est pas programmé pour fournir des réponses explicites ou catégoriques à vos questions. Il transforme plutôt vos questions en demandes API qui sont distribuées aux différents services informatiques officiellement administrés par l'EPFL. Son but est uniquement de collecter et de recommander des références pertinentes à des contenus que vous pouvez explorer pour vous aider à répondre à vos questions.
For a comparison with the corresponding alpha- and beta-hexapeptides H-(Val-Ala-Leu)2-OH (A) and H-(beta-HVal-beta-HAla-beta-HLeu)2-OH (B), the corresponding gamma-hexapeptide (I), built from the homochirally similar (S)-4-aminobutanoate, (R)-4-amino-5-met ...
A pore-forming peptide is implicated in the potent cytolytic activity of pathogenic Entamoeba histolytica. Using NH2-terminal sequence information of this peptide, the corresponding cDNA was isolated. The cDNA-deduced amino acid sequence revealed a putativ ...
The retention behaviour of a six-helix bundle template-assembled synthetic protein (TASP) molecule and its amphiphilic building blocks was investigated. The TASP consists of a circular template, cyclo(1-12)[KG]6, and six identical potentially alpha-helical ...
The RFX DNA binding domain is a novel motif that has been conserved in a growing number of dimeric DNA-binding proteins, having diverse regulatory functions, in eukaryotic organisms ranging from yeasts to humans. To characterize this novel motif, we have p ...
A serotonin 5-HT3 receptor was functionally expressed to high levels and on a large scale in mammalian cells with the Semliki Forest virus system. Conditions were optimized to maximize detergent solubilization of the receptor, while preserving ligand bindi ...
beta-Hexapeptides and a beta-dodecapeptide with sequences contg. two different types of beta-amino acids, aliph. proteinaceous side-chains in 2- or 3-position, were studied with CD and NMR measurements. With one exception, the secondary structures formed b ...
Proteolytic activation, oligomerization, and membrane insertion are three steps that precede channel formation by the bacterial toxin aerolysin. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and hydrogen-deuterium exc ...
Conformational changes occurring upon membrane binding and subsequent insertion of staphylococcal alpha-toxin were studied using complementary spectroscopic techniques. Experimental conditions were established where binding could be uncoupled from membrane ...
The purification to homogeneity of an active soluble 25 kDa fragment of CD23, produced in insect cells using the baculovirus expression system, is described. Peptide mapping and analysis by Edman degradation and mass spectrometry permitted partial characte ...
Aerolysin is a channel-forming protein secreted as a protoxin by Aeromonas hydrophila. Analytical centrifugation measurements showed that proaerolysin is a dimer in solution, and this was confirmed by chemical cross-linking with dimethyl suberimidate. Diss ...
