Comparison of the conformation and orientation of alamethicin and melittin in lipid membranes
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Pore-forming toxins, such as colicin A, are water-soluble proteins that insert into lipid bilayers. The water-soluble structure of Colicin A is known at a high resolution and this review describes the kinetic and structural steps involved in its soluble-to ...
Time-resolved fluorescence anisotropy (FA) measurements are reported for 5 helical, bilayer-spanning heneicosapeptides, each contg. 1 tryptophan (Trp) at sequence positions 1, 6, 11, 16, and 21, resp. The FA decay reflected 2 mol. processes in all cases, l ...
Monomolecular layers of lipid extracts of microsomal, mitochondrial outer and inner membranes, and pure lipid species have been used to measure their interaction with apo- and holocytochrome c. Large differences were observed both with respect to the natur ...
American Society for Biochemistry and Molecular Biology1989
Time-resolved fluorescence anisotropy (FA) measurements are reported for 5 helical bilayer-spanning henicosapeptides, each contg. 1 tryptophan at sequence position 1, 6, 11, 16, or 21. The FA decay reflects 2 mol. processes in all cases: local internal flu ...
Oligomerization is a necessary step in channel formation by the bacterial toxin aerolysin. We have identified a region of aerolysin containing two tryptophans which influence the ability of the protein to oligomerize. Changing the tryptophan at position 37 ...
Among several effects, ethanol (EtOH) interferes with membrane fluidity and lipid-protein interactions. As proteins are influenced by surrounding lipids, the activity of membrane-bound enzymes such as adenylate cyclase (AC) could be modulated by EtOH, as s ...
The secondary structure of bacterio-opsin (BO), the retinal free protein-component of bacteriorhodopsin (BR), was detd. by Raman spectroscopy. Addnl. CD measurements revealed only negligible conformational differences between BO in apomembranes and BR in p ...
In a recent paper, a new class of synthetic lipids, which self-assemble to form monolayers on gold surfaces, was introduced. The addn. of a monolayer of phospholipids to these layers results in robust supported lipid bilayers designed to reconstitute trans ...
Template-assembled proteins (TASPs) comprising 4 peptide blocks, each of either the natural melittin sequence (melittin-TASP) or of a truncated melittin sequence (amino acid 6-26, melittin6-26-TASP), C-terminally linked to a (linear or cyclic) 10-amino aci ...
Putative transmembrane helixes of membrane proteins in general and channel proteins in particular often contain proline residues which may induce a bend into an otherwise regular helical structure. Here, it is shown by fluorescence-energy-transfer measurem ...