Pf3 coat protein forms voltage-gated ion channels in planar lipid bilayers
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The goal of the present study is to construct a biophysical model of the coronary artery endothelial cell response to bradykinin. This model takes into account intracellular Ca2+ dynamics, membrane potential, a non-selective cation channel, and two Ca(2+)- ...
Three alkali cations, potassium, sodium, and lithium, have been separated within 15 s in a 1 cm long polymer microchip. The separation microchannel is modified by a polycation, poly(allylammonium chloride), which makes the channel surfaces positively charg ...
A recent study on malaria-infected human red blood cells (RBCs) has shown induced ion channel activity in the host cell membrane, but the questions of whether they are host- or parasite-derived and their molecular nature have not been resolved. Here we rep ...
The influence of external currents on the potential response of polymeric membrane ion-selective electrodes (ISEs) is analyzed theoretically. The treatment is based on the well-established potential model of ISE membranes, extended by contributions from cu ...
A method is presented to form gold-electrode-tethered lipid bilayers with exceptionally high elec. resistances. Elec. impedance spectroscopy is used to monitor the bilayer incorporation of a ligand-gated ion channel protein and the modulation of its channe ...
Aerolysin secreted by the human pathogen Aeromonas hydrophila belongs to a group of bacterial toxins that are hemolytic and form channels in biological membranes. The toxin is secreted as an inactive precursor proaerolysin that must be proteolytically proc ...
In this work the authors have shown that stable gigaohm seals over micrometer-sized holes can be obtained in a time-frame of seconds by the electrophoretic self-positioning of charged lipid membranes. For the first time, single-channel events were recorded ...
The functional anal. of single ion channel proteins presents a serious bottleneck in the process of finding new pharmacol. active compds. Currently available single channel recording methods are not suited for automation and miniaturization. However, new t ...
Serum amyloid A (SAA) is a small apolipoprotein that binds to high-density lipoproteins in the serum. Although SAA seems to play a role in host defense and lipid transport and metabolism, its specific functions have not been defined. Despite the growing im ...
Gating of an artificial receptor was monitored for the first time by impedance spectroscopy in tethered lipid bilayer. Subnanomolar concns. of antibody were detected by modulation of the channel activity. The principle of gating by an antibody is particula ...