Characterization of a transmembrane receptor protein by fluorescence correlation spectroscopy
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The ability of organisms, or individual cells, to react to external chemical signals, which are detected and transduced by cell-surface receptors, is crucial for their survival. These receptors are the targets of the majority of clinically used medicines. ...
We have expressed the 5-HT3 receptor with two transient expression sytems: the Semliki Forest virus and the HEK293EBNA. Both systems allow the rapid expression of functional receptor protein at very high level: up to 107 receptors per cell. Large scale pro ...
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A general method for understanding the mechanisms of ligand recognition and activation of G protein-coupled receptors has been developed. A study of ligand-receptor interactions in the prototypic seven-transmembrane neurokinin-2 receptor (NK2) using this f ...
G protein-coupled receptors (GPCRs) constitute an abundant family of membrane receptors of high pharmacol. interest. Cell-based assays are the predominant means of assessing GPCR activation, but are limited by their inherent complexity. Functional mol. ass ...
The nicotinic acetylcholine receptor from Torpedo was immobilised in tethered membranes. Surface plasmon resonance was used to quantify the binding of ligands and antibodies to the receptor. The orientation and structural integrity of the surface-reconstit ...
Two types of ligand-gated ion channels were expressed with the Semliki Forest virus (SFV) expression system. The cDNAs for mouse serotonin 5-HT3 receptor and rat and human purinoreceptor P2x subtypes were introduced into the pSFV1 vector. In vitro transcri ...
The pore-forming toxin aerolysin is secreted by Aeromonas hydrophila as an inactive precursor. Based on chemical cross-linking and gel filtration, we show here that proaerolysin exists as a monomer at low concentrations but is dimeric above 0.1 mg/ml. At i ...
The authors probed the function of receptor protein OmpF by in situ measurement of both the binding of ligands by surface plasmon resonance (SPR) and subsequent changes of the channel activity by impedance spectroscopy (IS). [on SciFinder (R)] ...
A bilayer lipid membrane sensor comprises (1) a gold recording surface; (2) a 1st lipid layer which is an imperfect layer of a thiolipid comprising the 2 phospholipid mols. linked to each end of a disulfide group, each through an oxyethylene chain short en ...
