Stable self-assembly of a protein engineering scaffold on gold surfaces
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The sequential condensation of peptide loops onto selectively addressable templates immobilised on solid supports allows the rapid and efficient synthesis of protein mimetics applying the TASP (Template Assembled Synthetic Protein) concept. As an example o ...
Membrane proteins fulfill many central functions in the biological membrane. The insertion process of these proteins and their structure, which are intimately linked to their function, are not yet well understood. As a model we studied three proteins recon ...
The reversible oriented immobilization of proteins on solid surfaces is a prerequisite for the investigation of mol. interactions at interfaces or the construction of supramol. assemblies. We demonstrate a generally applicable method using a synthetic chel ...
We demonstrate that the combined application of Langmuir-Blodgett and self-assembly techniques allows the fabrication of patterns with contrasting surface properties on gold substrates. The process is monitored using fluoroscence microscopy and surface pla ...
The formation of supported lipid mono- and bilayers on a thiol-coated surface was monitored by utilising the acoustic Love waveguide device. The detection of the lipid layers was used as a model system for relating the phase response to the deposited mass ...
Reversible and oriented immobilization of proteins in a functionally active form on solid surfaces is a prerequisite for the investigation of mol. interactions by surface-sensitive techniques. We demonstrate a method generally applicable for the attachment ...
A bilayer lipid membrane sensor comprises (1) a gold recording surface; (2) a 1st lipid layer which is an imperfect layer of a thiolipid comprising the 2 phospholipid mols. linked to each end of a disulfide group, each through an oxyethylene chain short en ...
The formation of supported lipid layers incorporating promastigote surface protease (PSP), a glycosylphosphatidylinositol-anchored protein, is investigated using surface plasmon resonance. Both hydrophilic and hydrophobic substrates are used for the format ...
In a recent paper, a new class of synthetic lipids, which self-assemble to form monolayers on gold surfaces, was introduced. The addn. of a monolayer of phospholipids to these layers results in robust supported lipid bilayers designed to reconstitute trans ...
A lipopeptide, carrying the antigenic peptide segment 135-154 of VP1, one of the capsid proteins of the picornavirus which causes foot-and-mouth disease in cattle, was investigated in lipid monolayers on the water surface and on hydrophobic solid supports. ...