Publication

Investigating cellular signaling reactions in single attoliter vesicles

Publications associées (87)

Dynamics of the 5-HT3A serotonin receptor in living cells

The 5-HT3 serotonin receptor belongs to the cys-loop ligand-gated ion channel (LGIC) family. It is located in the central and peripheral nervous system and is implicated in certain pathologic situations. The 5-HT3 receptor is investigated here, as a typica ...

EPFL2006

Investigating cellular signalling at the nanometer and attoliter scale

Horst Vogel

Spatial compartmentalisation is a prerequisite for the creation of living matter. Without the existence of clearly defined borders, differentiation and diversity at the cellular level would not be possible. Most scientific disciplines that deal with dissol ...

2005

Functional characterization of a human odorant receptor

Valérie Jacquier

Olfaction appears to be mediated by a large family of odorant receptors (ORs) that accounts for ~ 1% of the human genome [1–4]. The functional characterization of these receptors has so far been hindered by their poor functional expression in the plasma me ...

EPFL2005

Kinetics of the initial steps of G protein-coupled receptor-mediated cellular signaling revealed by single-molecule imaging

Horst Vogel, Bruno Meyer

We report on an in vivo single-mol. study of the signaling kinetics of G protein-coupled receptors (GPCR) performed using the neurokinin 1 receptor (NK1R) as a representative member. The NK1R signaling cascade is triggered by the specific binding of a fluo ...

2005

Noninvasive imaging of 5-HT3 receptor trafficking in live cells: From biosynthesis to endocytosis

Horst Vogel, Horst Pick, Cédric Deluz, Erwin Ilegems

Sequential stages in the life cycle of the ionotropic 5-HT3 receptor (5-HT3R) were resolved temporally and spatially in live cells by multicolor fluorescence confocal microscopy. The insertion of the enhanced cyan fluorescent protein into the large intrace ...

2004

Investigating cellular signalling reactions by micro- and nanotechnology

Horst Vogel

Classically, cellular signalling is investigated measuring different optical or elec. properties of either single cells or microliter vols. of suspensions of live cells. Here we show how ligand binding to cell surface receptors and subsequent signalling re ...

2004

Native membranes on subwavelentgh holes for membrane receptors analysis

Jürgen Brugger, Benjamin André René Meyer

How membrane receptors transduce external signals across the membrane and activate intracellular processes is a key question for fundamental research and for therapeutic purposes. Reliable in situ investigations requiest to preserve the full biological act ...

2004

Short-term immunosuppression reduces fibrotic cellular infiltration around barium-M-alginate microbeads injected intraportally

Philipp Bucher, Jutta Christine Wandrey

Introduction. One of the major barriers affecting the viability of encapsulated islets is pericapsular ﬁbrotic inﬁltration (PFI). This study aimed to design strategies to reduce PFI around intraportally injected alginate microbeads. Methods. Empty, highly ...

2004

Bioanalytical reagent, method for production thereof, sensor platforms and detection methods based on use of said bioanalytical reagent

Horst Vogel, Horst Pick

The invention relates to various embodiments of a bioanal. reagent with at least one vesicle, generated from a living cell, and at least one receptor for study of signal transduction. The invention further relates to methods for prodn. of said bioanal. rea ...

(Ecole Polytechnique Federale de Lausanne, Switz.; Zeptosens A.-G.).2002

Functional immobilisation of the nicotinic acetylcholine receptor in tethered lipid membranes

Horst Vogel, Rudolf Hovius, Per Rigler

The nicotinic acetylcholine receptor from Torpedo was immobilised in tethered membranes. Surface plasmon resonance was used to quantify the binding of ligands and antibodies to the receptor. The orientation and structural integrity of the surface-reconstit ...

Elsevier2000

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