Labeling of fusion proteins with synthetic fluorophores in live cells
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We report here the generation of mutants of the human O6-alkylguanine-DNA alkyltransferase (hAGT) for the efficient in vivo labeling of fusion proteins with synthetic reporter mols. Libraries of hAGT were displayed on phage, and mutants capable of efficien ...
Novel fluorescence approaches to investigate ligand recognition and structure of G protein-coupled receptors in native membranes have been developed. These methods combine the biosynthetic incorporation of unnatural fluorescent amino acids at known sites i ...
The specific and covalent labeling of fusion proteins with synthetic mols. opens up new ways to study protein function in the living cell. Here the authors present a novel method that allows for the specific and exclusive extracellular labeling of proteins ...
A review with 6 refs. of the authors' work in developing a biophys. technique to investigate the structure, function and dynamics of membrane receptors. A fluorescence-based approach has been developed and applied to the prototypic G protein-coupled recept ...
