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Since stem cells have the unique ability to produce more of themselves (i.e. to "self-renew") and to generate specialized tissue cells, they are an ideal source of cells for regenerative medicine and in vitro tissue models. In order to fully exploit this p ...
An unprecedented series of titanocene-gold bi- and trimetallic complexes of the general formula [(eta(5)-C5H5)-(mu-eta(5):kappa(1)-C5H4(CH2)(n)PPh2)TiCl2AuClx](q+) (n = 0, 2, or 4; m = 1, x =1, q = 0 or m = 2, x = 0, q = 1) have been prepared and char ...
The principal components and orientations of the chem. shift anisotropy (CSA) tensors of the carbonyl (C'), nitrogen (N), and amide proton (HN) nuclei of 64 distinct amide bonds in human ubiquitin have been detd. in isotropic soln. by a set of 14 complemen ...
A new strategy has been developed to measure cross-correlation rates with much enhanced accuracy. The method relies on the use of four complementary expts. Errors due to pulse miscalibration and to uncontrolled attenuation factors assocd. with relaxation a ...
Mutations in the parkin gene cause early-onset, autosomal recessive Parkinson's disease. Parkin functions as an E3 ubiquitin ligase to mediate the covalent attachment of ubiquitin monomers or linked chains to protein substrates. Substrate ubiquitination ca ...
We explored the relationship between ubiquitin proteasome system (UPS) and lysosomal markers and the formation of alpha-synuclein (alpha-syn) inclusions in nigral neurons in Parkinson disease (PD). Lysosome Associated Membrane Protein 1(LAMP1), Cathepsin D ...
R6/2 transgenic mice with expanded CAG repeats (> 300) have a surprisingly prolonged disease progression and longer lifespan than prototypical parent R6/2 mice (carrying 150 CAGs); however, the mechanism of this phenotype amelioration is unknown. We compar ...
Proteins of the endomembrane system undergo assisted folding in the endoplasmic reticulum (ER), then quality-control and, if misfolded, ER-associated degradation (ERAD). Recent findings on the biogenesis of a type-I membrane protein (an LRP6 mutant) lead u ...
A new technology for the specific and covalent labelling of fusion proteins with small organic molecules in vivo and in vitro has recently been developed in our laboratory. The method is based on the genetically encoded fusion of a target protein to O6-alk ...
α-Synuclein can be degraded by both the ubiquitin-proteasomal system and the chaperone-lysosomal system. However, the switching mechanism between the two pathways is not clearly understood. In our study, we investigated the mutual association between the b ...