Covalent labeling of cell-surface proteins for in-vivo FRET studies
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A fluorescent unnatural amino acid was introduced biosynthetically at known sites into the G protein-coupled neurokinin (tachykinin) NK2 receptor by suppression of UAG nonsense codons with the aid of a chem. misacylated synthetic tRNA specifically designed ...
Many central biol. signal transduction processes such as synaptic transmission occur at the level of cell membranes. It is therefore not surprising that the biol. function of membrane proteins in general and neuroreceptors in particular can be modulated by ...
During the membrane insertion process the major coat protein of bacteriophage M13 assumes a conformation in which two transmembrane helixes corresponding to the leader sequence and the anchor region in the mature part of the protein coming into close conta ...
A fluorescently labeled ligand for the 5-HT3 serotonin receptor was synthesized and its sub-nanomolar affinity for the purified, detergent solubilized receptor was measured. The change in the ligand's fluorescence upon receptor binding was used to directly ...
A review with 6 refs. of the authors' work in developing a biophys. technique to investigate the structure, function and dynamics of membrane receptors. A fluorescence-based approach has been developed and applied to the prototypic G protein-coupled recept ...
A general method for understanding the mechanisms of ligand recognition and activation of G protein-coupled receptors has been developed. A study of ligand-receptor interactions in the prototypic seven-transmembrane neurokinin-2 receptor (NK2) using this f ...
A highly-sensitive surface fluorescence technique was combined with a powerful method for the oriented reversible immobilization of membrane proteins on oxide surfaces. Affinity and kinetic parameters of the interaction of the receptor and a fluorescent li ...
Fluorescence anisotropy measurements on isotropically distributed membranes yield the well-known orientational order parameter as a measure of orientational fluctuations of the fluorophore that are fast compared to the fluorescence lifetime. Measurements o ...
The screening of ligands for membrane receptor proteins is central to the discovery of new pharmaceutical drugs. We present a general method to reversibly attach receptor proteins via an affinity tag to a quartz surface and subsequently detect with high se ...