Total internal reflection fluorescence correlation spectroscopy (TIR-FCS) with low background and high count-rate per molecule
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High capacity data storage on DVD and optical discs is based on focused light beams. The characterization of light fields within the focus point is a very difficult task, which requires a high-resolution method like scanning near field optical microscopy ( ...
The present thesis develops some specific aspects of digital holographic microscopy (DHM), namely the effect of shot noise on the phase image accuracy, the use of DHM in micro-tomography and in aberrations evaluation of a microscope objective (MO). DHM is ...
Monitoring biological relevant reactions on the single molecule level based on fluorescence spectroscopy techniques has become one of the most promising approaches for understanding a variety of phenomena in biophysics, biochemistry and life science. By ap ...
In this thesis a new approach for single molecule detection and analysis is explored. This approach is based on the combination of two well established methods, fluorescence correlation spectroscopy (FCS) and total internal reflection fluorescence microsco ...
With the recent development of fluorescent probes and new high-resolution microscopes, biological imaging has entered a new era and is presently having a profound impact on the way research is being conducted in the life sciences. Biologists have come to d ...
The evolution path of nanotechnologies moves through the domain of microscopy. Among all the kinds of imaging tools available today, optical imaging systems are of primary importance. When using optical imaging systems, we take profit of the information re ...
Institut de Microtechnique, Université de Neuchâtel2006
In this study a novel approach for the coating and functionalization of substrates for cell culture and tissue engineering is presented. Glass, silicon, and titanium panes were coated with an ultrathin film (30 ± 5 nm) of reactive star-shaped poly(ethylene ...
This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. Objective-type TIR-FCS combines ...
We achieved photon count rates per molecule as high as with commonly used confocal fluorescence correlation spectroscopy instruments using a new total internal reflection fluorescence correlation spectroscopy system based on an epi-illumination configurati ...
Fluorescence fluctuation spectroscopy based on an evanescent field excitation scheme leads to an alternative concept for single molecule detection with interesting experimental features. This concept is described and analyzed regarding the total fluorescen ...