Production of lactate using crabtree negative organisms expressing recombinant lactate dehydrogenase genes
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Lactate concentration in physiological fluids is an indicator of the energy production under anaerobic conditions of the whole organism [1]. Normal value for lactate level in blood ranges from 0.5 – 2.5 mM. Blood lactate values exceeding 7-8 mM are sometim ...
Interfaces are important for many processes in chemistry, physics, and biology. Crucial to their properties are the details of the three-dimensional structure of the participating (macro)molecules. Vibrational Sum Frequency Generation (VSFG) is a tool spec ...
To date, functional 1H NMR spectroscopy has been utilized to report the time courses of few metabolites, primarily lactate. Benefiting from the sensitivity offered by ultra-high magnetic field (7 T), the concentrations of 17 metabolites were measured in th ...
The objective of this study was to develop a model for the growth of Chinese Hamster Ovary (CHO) cells in shaken bioreactors. For this purpose a modified three parameters Gompertz equation was used to fit lag, exponential and stationary growth phases. Usin ...
BACKGROUND: For a long time now, glucose has been thought to be the main, if not the sole substrate for brain energy metabolism. Recent data nevertheless suggest that other molecules, such as monocarboxylates (lactate and pyruvate mainly) could be suitable ...
The monitoring of metabolic compounds such as glucose is largely reported in literature. The applications of this type of analysis are mainly related to clinical purposes, e.g. in diabetes pathology, where a lot of studies are presented in literature. Rece ...
Animal cell (Chinese Hamster Ovary) concn. was detd. online in a packed bed process using dielec. spectroscopy. This enabled the evaluation of the effect of temp. on specific metabolic rates during 3 mo of continuous culture. The effect of low cultivation ...
Flash photolysis of caged compounds is one of the most powerful approaches to investigate the dynamic response of living cells. Monolithically integrated devices suitable for optical uncaging are in great demand since they greatly simplify the experiments ...
Bioresorbable scaffolds made Of poly(L-lactic acid) (PLA) obtained by supercritical gas foaming were recently described as suitable for tissue engineering, portraying biocompatibility with primary osteoblasts in vitro and interesting mechanical properties ...
This report describes the synthesis and supramolecular organization of a novel class of linear-dendritic block copolymers. The molecules, which are termed rodcoil dendrons, consist of a cholesterol moiety that is attached to L-lysine dendrons of three diff ...