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This lecture explores the dynamics of organelles, focusing on the relationship between mitochondria and lipid droplets. It discusses the limitations of current imaging techniques due to phototoxicity and introduces the 3D Cell Explorer as a non-invasive tool to overcome these issues. The lecture showcases time-lapse videos of pre-adipocytes imaged with high resolution, highlighting the interactions between mitochondria and lipid droplets. It also delves into the composition of organelles, the variability in morphology, and the transport mechanisms of lipids and proteins within cells.