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This lecture covers the hierarchy of hormones in the body, starting from the hypothalamus down to the pancreas and kidneys. It explains how insulin and glucagon regulate blood glucose levels through intricate mechanisms involving various organs and signaling pathways. The lecture delves into the synthesis and processing of insulin, the role of pancreatic beta cells in glucose sensing, and the molecular processes behind insulin exocytosis. Additionally, it explores the effects of insulin and glucagon on glucose metabolism in different tissues, as well as the pathophysiology of type 1 and type 2 diabetes.