Biochemical Reactions: Equilibrium and Spontaneity

This lecture focuses on the equilibrium and spontaneity of biochemical reactions, using the example of the transformation of 3PGA into 2PGA. The instructor explains the calculations of standard Gibbs free energy, reaction quotients, and the impact of changing conditions on reaction directionality. The discussion extends to cellular equilibrium, emphasizing the importance of concentration gradients for cell function. Through detailed examples and equations, the lecture illustrates how reactions shift between spontaneity and equilibrium based on varying conditions, both in vivo and in vitro. The application of thermodynamic principles to biochemical systems is highlighted, showcasing the dynamic nature of cellular processes.