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This lecture focuses on exercise 6, discussing the formation and transformation of 3-phosphoglycerate (3 PGA) into 2-phosphoglycerate (2 PGA) under equilibrium conditions. The instructor explains the importance of standard biological conditions and the calculation of standard Gibbs free energy. The lecture also covers the concept of cellular equilibrium, the role of cofactors in biochemical reactions, and how to manipulate reaction directionality by adjusting reactant concentrations.