Cell Adhesion: Integrin Signaling and ECM Mechanics

This lecture delves into the mechanisms of cell adhesion, focusing on integrin signaling and extracellular matrix (ECM) mechanics. It covers how actin structures are linked to focal adhesions, the role of well-known signaling molecules in mechano-transduction, and the impact of synthetic adhesion peptides like RGD. The discussion extends to the effects of substrate stiffness on cell behavior and stem cell differentiation, emphasizing the sensitivity of mesenchymal stem cells to tissue-level elasticity. Additionally, the lecture explores the use of Matrigel in cell culture and the advantages of synthetic ECM scaffolds over Matrigel in providing a defined and reproducible environment for stem cell culture and tissue engineering.