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This lecture covers the main physiological measurands, focusing on biopotentials such as Electroneurogram (ENG) and Electromyogram (EMG). It explains the characteristics of these signals, including amplitude, bandwidth, and measurement techniques. Additionally, it delves into the interpretation of biopotentials in medical instrumentation, highlighting the importance of sensors in capturing these signals accurately. The lecture also explores the Electrocardiogram (ECG) and Electroencephalogram (EEG), discussing the different electrode types used for brain-level biopotential observation. It further examines the frequency bands associated with EEG signals during awake states, intense mental activity, emotional states, and deep sleep.