Lecture

Intensity and Localization Analysis

In course

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Description

This lecture covers the analysis of intensity and localization in a dataset of images with three fluorescent widefield channels acquired for three conditions (Wells 06, 08, 11). The microscope used outputs individual tiff images representing DAPI staining, Phalloidin (AlexaF488), and a Red Fluorescent protein fused to the protein of interest. The main goal is to measure the change in the expression of the protein of interest across different conditions.

Instructors (4)

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Official source

https://mediaspace.epfl.ch/media/0_mrfcvvx3

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