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This lecture covers the principles and workflows of Cryo-Electron Microscopy (Cryo-EM) in life sciences, focusing on imaging techniques, sample preparation, and data processing. It discusses the resolution revolution in Cryo-EM, the workflow of a Cryo-EM project, and the current state of Cryo-EM technology. The lecture also explores the application of Cryo-EM in single-particle analysis, atomic structure determination, and method development. Additionally, it highlights the role of microfluidics in Cryo-EM for sample manipulation and analysis.