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This lecture explores the critical process of carbon fixation, where CO₂ is converted to organic carbon by primary producers, providing food and energy for the planet. It covers the pathways used by various organisms, such as the Calvin-Benson-Bassham cycle and the reductive citric acid cycle, highlighting the key enzymes and intermediates involved. The lecture also delves into the importance of autotrophs capable of carbon fixation, including cyanobacteria, green sulfur bacteria, and archaea, in different environments. Additionally, it discusses the role of ATP citrate lyase and the reductive acetyl-coA pathway in the conversion of CO₂ to acetyl-coA. Overall, the lecture provides a comprehensive overview of the mechanisms and significance of carbon fixation in sustaining life on Earth.