Protein Separation Techniques

This lecture covers various protein separation techniques, including polyacrylamide gel electrophoresis, SDS-PAGE, 1D and 2D gel electrophoresis, isoelectric focusing, and chromatography. It explains the principles behind each technique, such as pore size, charge, and molecular mass, and their applications in proteomics. The lecture also discusses the importance of factors like pH, electric charge, and hydrophobicity in protein separation.