Proteomics and Mass Spectrometry

This lecture covers the principles and techniques of protein mass spectrometry and proteomics, focusing on gel electrophoresis, isoelectric focusing, and liquid chromatography. It explains the importance of protein separation, sampling, and preparation, highlighting the need for separation due to the diversity of protein sequences and concentrations. The lecture also discusses the use of tandem mass spectrometry for protein identification and the generation of tandem mass spectra. Various electrophoresis set-ups, polyacrylamide gel polymerization, and 1D SDS PAGE techniques are explained in detail, along with the properties of amino acids and their role in isoelectric focusing. The lecture concludes with a discussion on the charge of polypeptides and the generation of pH gradients for protein separation.