Exploring Proteins: Structure, Purification, and Analysis

This lecture introduces the exploration of proteins and proteomes, covering topics such as protein synthesis, composition, structure, and diversity. It delves into protein purification methods like ion exchange chromatography and gel-filtration chromatography, as well as techniques for protein analysis including mass spectrometry and electrophoresis. The lecture also discusses protein sequencing, concentration measurement, and the use of antibodies for protein detection. Furthermore, it explains the three-dimensional structure determination of proteins through x-ray crystallography and highlights the importance of isoelectric focusing and high-pressure liquid chromatography in protein analysis.