Experimental Biochemistry and Biophysics: Genetic Engineering and FRET Sensors

This lecture covers the practical aspects of experimental biochemistry and biophysics, focusing on genetic engineering and the characterization of a FRET sensor of enzymatic activity. Students will learn about DNA manipulation techniques, protein expression, purification, and characterization, as well as the kinetics of enzymatic reactions. The lecture also emphasizes the importance of lab safety and the differences between bio-labs and chemistry labs. Practical skills such as planning, teamwork, and lab journaling are highlighted, preparing students for future projects in chemical biology. The session concludes with a discussion on the evaluation criteria and the final report submission.