Chemical Biology Tools: DDX3 Mutant Analysis

This lecture delves into the analysis of a mutant DDX3 enzyme, focusing on its ATPase activity and the development of an analog-sensitive inhibitor. It covers concepts like RNA chaperones, crystal structures, hydrophobic residues, and dose-response traces. The lecture also explores mass spectrometry-based protein profiling techniques, including bottom-up and top-down approaches, peptide digestion, and affinity pulldown methods. Additionally, it discusses stable isotope labeling for quantitative target identification and activity-based protein profiling for rapid proteome analysis.