Illumination in Microscopy

This lecture introduces the concept of illumination in microscopy, covering critical illumination, Kohler illumination, conjugated planes, and the infinity corrected compound microscope. It explains the importance of field or image forming conjugate planes, the role of aperture in illuminating conjugate planes, and the differences between critical and Kohler illumination methods. The instructor discusses the historical background of August Koehler's illumination system and its impact on image quality. The lecture also addresses the limitations of conventional compound microscopes and the advantages of infinity corrected compound microscopes in producing parallel light wavefronts. Furthermore, it explores the significance of conjugate planes in the context of infinity corrected microscopes.