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This lecture covers the historical experience of immunization with ovalbumin, the development of monoclonal antibodies by Koehler and Milstein, the synthesis of purines and pyrimidines, the structure of antibodies, antigen recognition, and the characteristics of antigens. It explains the process of hybridoma formation, the function of monoclonal antibodies, and the diversity in the variable region. Additionally, it discusses the types of antigens, including self antigens, allogenic antigens, and haptens, as well as the flexibility and valence of antigen recognition.