Publication
In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of beta 2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells.
Giovanni D'Angelo, Laura Capolupo, Anthony Vocat, Riccardo Rizzo
Maartje Martina Cornelia Bastings, Diana Kornelia Morzy
Philippe Renaud, Horst Vogel, Thamani Dahoun, Margaux Catherine Marie Duchamp