Concept
Site-specific recombination
Related publications (36)
Vibrio cholerae pathogenicity island 2 encodes two distinct types of restriction systems
Melanie Blokesch, David William Adams, Alexandre Lemopoulos, Grazia Vizzarro
In response to predation by bacteriophages and invasion by other mobile genetic elements such as plasmids, bacteria have evolved specialised defence systems that are often clustered together on genomic islands. The O1 El Tor strains of Vibrio cholerae resp ...
2024Robust designation of meiotic crossover sites by CDK-2 through phosphorylation of the MutS gamma complex
Crossover formation is essential for proper segregation of homologous chromosomes during meiosis. Here, we show that Caenorhabditis elegans cyclin-dependent kinase 2 (CDK-2) partners with cyclin-like protein COSA-1 to promote crossover formation by promoti ...
NATL ACAD SCIENCES2022The makings of TERRA R-loops at chromosome ends
Joachim Lingner, Marianna Feretzaki, Rita Valador Fernandes
Telomeres protect chromosome ends from nucleolytic degradation, uncontrolled recombination by DNA repair enzymes and checkpoint signaling, and they provide mechanisms for their maintenance by semiconservative DNA replication, telomerase and homologous reco ...
TAYLOR & FRANCIS INC2021m6A methylation potentiates cytosolic dsDNA recognition in a sequence-specific manner
Andrea Ablasser, Muhammet Fatih Gülen
Nucleic acid sensing through pattern recognition receptors is critical for immune recognition of microbial infections. Microbial DNA is frequently methylated at the N-6 position of adenines (m6A), a modification that is rare in mammalian host DNA. We show ...
ROYAL SOC2021Site-Specific Protein Conjugation onto Fluorescent Single-Walled Carbon Nanotubes
Ardemis Anoush Boghossian, Shang-Jung Wu, Vitalijs Zubkovs
Semiconducting single-walled carbon nanotubes (SWCNTs) are among the few photostable optical emitters that are ideal for sensing, imaging, drug delivery, and monitoring of protein activity. These applications often require strategies for immobilizing prote ...
2020Mitotic chromosome binding predicts transcription factor properties in interphase
David Michael Suter, Cédric Deluz, Torgil Elias Friman, Andrea Brigitta Alber, Mahé Cécile Raccaud
Mammalian transcription factors (TFs) differ broadly in their nuclear mobility and sequence-specific/non-specific DNA binding. How these properties affect their ability to occupy specific genomic sites and modify the epigenetic landscape is unclear. The as ...
2019MAR-Mediated transgene integration into permissive chromatin and increased expression by recombination pathway engineering
Christian Iseli, Nicolas Mermod, Thomas Junier, Samuel Neuenschwander, Ioannis Xenarios
Untargeted plasmid integration into mammalian cell genomes remains a poorly understood and inefficient process. The formation of plasmid concatemers and their genomic integration has been ascribed either to non-homologous end-joining (NHEJ) or homologous r ...
2017