Concept

Fluorescence microscope

Related publications (797)

Waveguide-Based Platform for Large-FOV Imaging of Optically Active Defects in 2D Materials

Aleksandra Radenovic, Suliana Manley, Vytautas Navikas, Martina Lihter, Evgenii Glushkov, Anton Stroganov, Mukeshchand Thakur, Anna Archetti, Juan Francisco Gonzalez Marin, Jean Camille Benjamin Comtet

Single-molecule localization microscopy (SMLM) is a powerful tool that is routinely used for nanoscale optical imaging of biological samples. Recently, this approach has been applied to study optically active defects in two-dimensional (2D) materials. Such ...

AMER CHEMICAL SOC2019

Manipulation of redox signaling in mitochondria with trialkylphosphine chemical probes

Pablo Rivera Fuentes

Subcellular organelles maintain distinct redox environments to carry out their normal physiol. functions. Disruption of redox homeostasis, either in the oxidative or reductive direction, is linked to disease. We recently showed that trialkylphosphine-based ...

American Chemical Society2019

Waveguide platform and methods for super-resolution fluorescence microscopy of sub-cellular structures

Anna Archetti

Super-resolution fluorescence microscopy is widespread, owing to its demonstrated ability to resolve dynamical processes within cells and to identify the structure and position of specific proteins in the interior of protein complexes. Nowadays, subcellula ...

EPFL2019

Black Lipid Membranes: Challenges in Simultaneous Quantitative Characterization by Electrophysiology and Fluorescence Microscopy

Esther Amstad, Stefan Matile, Maria Tsemperouli

Horizontal black lipid membranes (BLMs) enable optical microscopy to be combined with the electrophysiological measurements for studying ion channels, peptide pores, and ionophores. However, a careful literature review reveals that simultaneous fluorescenc ...

AMER CHEMICAL SOC2019

Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence

Christian Depeursinge, Pierre Marquet, Chen Liu, Shanshan Kou

We report a dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional (3D) space. By introducing a direct way of deriving the 3D scattering potential of the object ...

2019

Preexisting variation in DNA damage response predicts the fate of single mycobacteria under stress

John McKinney, Neeraj Dhar, Giulia Manina, Lalit Kumar Singh

Clonal microbial populations are inherently heterogeneous, and this diversification is often considered as an adaptation strategy. In clinical infections, phenotypic diversity is found to be associated with drug tolerance, which in turn could evolve into g ...

2019

Photons in - numbers out: perspectives in quantitative fluorescence microscopy for in situ protein counting

Kristin Stefanie Grussmayer

The full understanding of cellular functions requires information about protein numbers for various biomolecular assemblies and their dynamics, which can be partly accessed by super-resolution fluorescence microscopy. Yet, many protein assemblies and cellu ...

2019

Photoregulated fluxional fluorophores for live-cell super-resolution microscopy with no apparent photobleaching

Pablo Rivera Fuentes

Photoswitchable mols. have multiple applications in the phys. and life sciences because their properties can be modulated with light. Fluxional mols., which undergo rapid degenerate rearrangements in the electronic ground state, also exhibit switching beha ...

Springer2019

Combining fluorescence-based image segmentation and automated microfluidics for ultrafast cell-by-cell assessment of biomarkers for HER2-type breast carcinoma

Martinus Gijs, Daniel Migliozzi, Huu Tuan Nguyen

Immunohistochemistry (IHC) is one of the main clinical techniques for biomarker assessment on tissue biopsies. It consists in chromogenic labeling with specific antibodies, followed by optical imaging, and it is used for diagnosis and therapeutic targeting ...

SPIE-Intl Soc Optical Eng2019

Photoactivation of silicon rhodamines via a light-induced protonation

Kai Johnsson, Luc Reymond, Michelle Susanne Frei

Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to ...

2019

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